长三角地区某市垃圾焚烧行业环保现状调查研究

对长三角地区某市垃圾焚烧行业的环保现状进行了调查研究,结果发现:当前区域垃圾焚烧企业,在保证规模、控制数量及地域上均衡布置的前提下,行业废水、废气污染治理设施齐全,废水、废气均稳定可达标排放,调查时段内未有投诉问题。区域垃圾焚烧行业在做到当下要求的情况下,实现了区域垃圾焚烧行业污染可控,对城市垃圾焚烧行业环保发展具备借鉴意义。     

羊支原体性肺炎两标准株对抗菌药物敏感性的研究

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。     

成龄山楂密植园低产改造技术

自2002年开始,对1985年定植、株行距2m×3m的低产山楂园进行改造,改造后产量逐年上升。2003年666.7m2产量1254kg,2004年2212kg,2005年达2635kg,比改造前(2002年)增产3倍多,经济收入增加6倍以上。主要技术措施:深翻扩穴,浅刨树盘,增施有机肥,及时追肥浇水,全园覆草,合理间伐,疏大枝,回缩冗长枝,冬剪结合培养紧凑枝组充实内膛,落头开心控制树高,刮树皮,疏花序,花期喷施山楂丰收素提高坐果率和及时防治病虫害等。     

黄淮夏大豆主要农艺性状的遗传分析

利用黄淮地区的12个高产夏大豆品种(系),通过NCⅡ交配设计,对12个主要农艺性状的遗传进行了分析。结果表明,所有性状的加性方差大于显性方差。主茎节数、株高、每簇荚数和主茎荚数的加性成分占绝对优势,遗传力较高。分枝荚数、单株粒数和单株粒重的显性成分比例大,遗传力较低,其中单株粒重趋于完全显性。主茎节数的显性成分很少,其它性状均为部分显性。建立了单株产量与产量因子之间的逐步回归方程。     

中国葡萄野生种对葡萄根癌病的抗性

通过田间接种鉴定，系统研究了中国葡萄属１３个种和变种的２６个株系对葡萄根癌病的抗性，筛选出高抗株系１０个。其中燕山葡萄（♀）因扦插容易生根，可用自根苗作为抗葡萄根癌病砧木进行嫁接试验，其余可用作抗葡萄根癌病的育种资源     

海藻肥对黄瓜种子萌发盐胁迫伤害的缓解作用

盐胁迫会破坏种子细胞的结构和功能,导致代谢紊乱,活力降低乃至失去活力。李庭红认为在15g·L-1NaCl盐胁迫下,黄瓜种子的发芽率一般为50%-60%。前人曾以乙烯利、矮壮素、水杨酸等溶液浸种的方法缓解盐胁迫对萌发种子的伤害,但成本高,操作繁琐,效果也不尽理想。海藻肥是一种从海藻中提取的天然生长调节     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

农杆菌介导的蝴蝶兰基因转化系统的建立

 针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB)，与含绿色荧光蛋白基因( )和潮霉素磷酸转移酶基因(^p￡)的pCAMBIA 1300一SmGFP的根瘤农杆菌LBA4404 共培养，培育出了转基因的蝴蝶兰。经绿色荧光蛋白检测和Southern印迹，证实了再生植株中含cop基因和hpt基因。     

Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing

microRNA (miRNA) and piwi‐interacting RNA (piRNA) are two classes small non‐coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next‐generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatogenesis. In conclusion, this study provided a useful resource for further elucidation of the miRNAs and piRNAs regulatory roles in spermatogenesis. It may also facilitate the development of therapeutic strategies for dzo reproduction research.     

苜蓿抗旱生理与分子机制

苜蓿(Medicago sativa)是我国乃至全世界最优质的豆科牧草之一,具有较高的经济价值、营养价值和生态价值。然而我国的苜蓿种植大多集中在干旱半干旱的西北、东北一带,水分是影响其产量的主要因素。本文通过对比光合呼吸系统、活性氧防御系统、渗透调节系统、脱落酸等在干旱胁迫下的变化分析了苜蓿抵御干旱胁迫的生理机制,同时对转录因子、蛋白酶基因和抗旱基因在苜蓿干旱胁迫下的应答过程进行了阐述,并对未来苜蓿应答干旱胁迫的研究重点进行了展望。